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blasticidin resistance gene  (AMS Biotechnology)


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    AMS Biotechnology blasticidin resistance gene
    Blasticidin Resistance Gene, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blasticidin+resistance+gene/CMV-Luciferase+(firefly)-2A-GFP+(Bsd)%2C+in+vivo+ready/pm41965340-185-21-24
    Average 96 stars, based on 2 article reviews
    blasticidin resistance gene - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Luciferase:

    Article Title: Therapy-induced normal tissue damage promotes breast cancer metastasis
    Article Snippet: .. Lucifierase (firefly) lentiviral particles, firefly luciferase gene with a blasticidin-resistance gene , Amsbio , Cat#LVP326. .. PGK-H2BmCherry-IRIS-Luc2 , Genscript , N/A.

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: To generate Raji (ATCC, CCL-86) and Toledo (ATCC, CRL-2631) knockout cell lines, Cas9 ribonucleoproteins (RNPs) were prepared by incubating 60 ng of Cas9 (IDT, 1081059) with 35 pmol sgRNA (Synthego) in Neon electroporation buffer (Invitrogen, MPK1096E). .. Luciferase (Luc)-Green Fluorescent Protein (GFP)-expressing Raji cells, generated by transduction with an LVV encoding firefly luciferase, Tag GFP 2, and the Blasticidin resistance gene (AMSbio, LVP323PBS), were mixed with Cas9 RNPs and electroporated with a Neon transfection kit (Invitrogen, MPK1096) and device (Invitrogen, MPK5000). .. Transfected cells were expanded in RPMI 1640 medium (Gibco, 72400-047) containing 10% FBS (GE Healthcare, SH300.70.03) and KO cells were isolated by FACS.

    Article Title: Generation and characterisation of two D2A1 mammary cancer sublines to model spontaneous and experimental metastasis in a syngeneic BALB/c host
    Article Snippet: .. Cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). ..

    Article Title: Therapy-induced senescence in normal tissue promotes breast cancer metastasis
    Article Snippet: Cyclophosphamide monohydrate was purchased from Sigma-Aldrich (C0768). .. As indicated, tumour cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (Genescript, Sall-IRES-Luc2-XbalXhol_PGK-H2BmCherry); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry+/RFP+ cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Therapy-induced normal tissue damage promotes breast cancer metastasis
    Article Snippet: .. As indicated, tumor cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (PGK-H2BmCherry-IRIS-Luc2; Genscript); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry + /RFP + cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
    Article Snippet: .. 4T1, D2A1 and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). .. Where indicated, 4T1-Luc cells were transduced with lentiviral particles expressing H2B-mRFP as previously described as well as D2A1, D2A1-m12 and MDA-MB-453 cells with a luciferase-mCherry vector. mCherry+/RFP+ cells were enriched by fluorescence-activated cell sorting (FACS).

    Article Title: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
    Article Snippet: E0771 cells were purchased from CH3 BioSystems. .. 4T1, D2A1, and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). .. Where indicated, 4T1-Luc cells were transduced with lentiviral particles expressing H2B-mRFP as previously described as well as D2A1, D2A1-m12, and MDA-MB-453 cells with a luciferase2-mCherry vector (Luc2-mCh). mCherry+/RFP+ cells were enriched by fluorescence-activated cell sorting (FACS).

    Generated:

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: To generate Raji (ATCC, CCL-86) and Toledo (ATCC, CRL-2631) knockout cell lines, Cas9 ribonucleoproteins (RNPs) were prepared by incubating 60 ng of Cas9 (IDT, 1081059) with 35 pmol sgRNA (Synthego) in Neon electroporation buffer (Invitrogen, MPK1096E). .. Luciferase (Luc)-Green Fluorescent Protein (GFP)-expressing Raji cells, generated by transduction with an LVV encoding firefly luciferase, Tag GFP 2, and the Blasticidin resistance gene (AMSbio, LVP323PBS), were mixed with Cas9 RNPs and electroporated with a Neon transfection kit (Invitrogen, MPK1096) and device (Invitrogen, MPK5000). .. Transfected cells were expanded in RPMI 1640 medium (Gibco, 72400-047) containing 10% FBS (GE Healthcare, SH300.70.03) and KO cells were isolated by FACS.

    Transduction:

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: To generate Raji (ATCC, CCL-86) and Toledo (ATCC, CRL-2631) knockout cell lines, Cas9 ribonucleoproteins (RNPs) were prepared by incubating 60 ng of Cas9 (IDT, 1081059) with 35 pmol sgRNA (Synthego) in Neon electroporation buffer (Invitrogen, MPK1096E). .. Luciferase (Luc)-Green Fluorescent Protein (GFP)-expressing Raji cells, generated by transduction with an LVV encoding firefly luciferase, Tag GFP 2, and the Blasticidin resistance gene (AMSbio, LVP323PBS), were mixed with Cas9 RNPs and electroporated with a Neon transfection kit (Invitrogen, MPK1096) and device (Invitrogen, MPK5000). .. Transfected cells were expanded in RPMI 1640 medium (Gibco, 72400-047) containing 10% FBS (GE Healthcare, SH300.70.03) and KO cells were isolated by FACS.

    Article Title: Generation and characterisation of two D2A1 mammary cancer sublines to model spontaneous and experimental metastasis in a syngeneic BALB/c host
    Article Snippet: .. Cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). ..

    Article Title: Therapy-induced senescence in normal tissue promotes breast cancer metastasis
    Article Snippet: Cyclophosphamide monohydrate was purchased from Sigma-Aldrich (C0768). .. As indicated, tumour cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (Genescript, Sall-IRES-Luc2-XbalXhol_PGK-H2BmCherry); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry+/RFP+ cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Therapy-induced normal tissue damage promotes breast cancer metastasis
    Article Snippet: .. As indicated, tumor cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (PGK-H2BmCherry-IRIS-Luc2; Genscript); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry + /RFP + cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
    Article Snippet: .. 4T1, D2A1 and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). .. Where indicated, 4T1-Luc cells were transduced with lentiviral particles expressing H2B-mRFP as previously described as well as D2A1, D2A1-m12 and MDA-MB-453 cells with a luciferase-mCherry vector. mCherry+/RFP+ cells were enriched by fluorescence-activated cell sorting (FACS).

    Article Title: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
    Article Snippet: E0771 cells were purchased from CH3 BioSystems. .. 4T1, D2A1, and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). .. Where indicated, 4T1-Luc cells were transduced with lentiviral particles expressing H2B-mRFP as previously described as well as D2A1, D2A1-m12, and MDA-MB-453 cells with a luciferase2-mCherry vector (Luc2-mCh). mCherry+/RFP+ cells were enriched by fluorescence-activated cell sorting (FACS).

    Transfection:

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: To generate Raji (ATCC, CCL-86) and Toledo (ATCC, CRL-2631) knockout cell lines, Cas9 ribonucleoproteins (RNPs) were prepared by incubating 60 ng of Cas9 (IDT, 1081059) with 35 pmol sgRNA (Synthego) in Neon electroporation buffer (Invitrogen, MPK1096E). .. Luciferase (Luc)-Green Fluorescent Protein (GFP)-expressing Raji cells, generated by transduction with an LVV encoding firefly luciferase, Tag GFP 2, and the Blasticidin resistance gene (AMSbio, LVP323PBS), were mixed with Cas9 RNPs and electroporated with a Neon transfection kit (Invitrogen, MPK1096) and device (Invitrogen, MPK5000). .. Transfected cells were expanded in RPMI 1640 medium (Gibco, 72400-047) containing 10% FBS (GE Healthcare, SH300.70.03) and KO cells were isolated by FACS.

    Expressing:

    Article Title: Generation and characterisation of two D2A1 mammary cancer sublines to model spontaneous and experimental metastasis in a syngeneic BALB/c host
    Article Snippet: .. Cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). ..

    Article Title: Therapy-induced senescence in normal tissue promotes breast cancer metastasis
    Article Snippet: Cyclophosphamide monohydrate was purchased from Sigma-Aldrich (C0768). .. As indicated, tumour cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (Genescript, Sall-IRES-Luc2-XbalXhol_PGK-H2BmCherry); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry+/RFP+ cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Therapy-induced normal tissue damage promotes breast cancer metastasis
    Article Snippet: .. As indicated, tumor cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (PGK-H2BmCherry-IRIS-Luc2; Genscript); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry + /RFP + cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
    Article Snippet: .. 4T1, D2A1 and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). .. Where indicated, 4T1-Luc cells were transduced with lentiviral particles expressing H2B-mRFP as previously described as well as D2A1, D2A1-m12 and MDA-MB-453 cells with a luciferase-mCherry vector. mCherry+/RFP+ cells were enriched by fluorescence-activated cell sorting (FACS).

    Article Title: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
    Article Snippet: E0771 cells were purchased from CH3 BioSystems. .. 4T1, D2A1, and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). .. Where indicated, 4T1-Luc cells were transduced with lentiviral particles expressing H2B-mRFP as previously described as well as D2A1, D2A1-m12, and MDA-MB-453 cells with a luciferase2-mCherry vector (Luc2-mCh). mCherry+/RFP+ cells were enriched by fluorescence-activated cell sorting (FACS).

    Fluorescence:

    Article Title: Therapy-induced senescence in normal tissue promotes breast cancer metastasis
    Article Snippet: Cyclophosphamide monohydrate was purchased from Sigma-Aldrich (C0768). .. As indicated, tumour cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (Genescript, Sall-IRES-Luc2-XbalXhol_PGK-H2BmCherry); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry+/RFP+ cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Therapy-induced normal tissue damage promotes breast cancer metastasis
    Article Snippet: .. As indicated, tumor cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (PGK-H2BmCherry-IRIS-Luc2; Genscript); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry + /RFP + cells were selected by fluorescence-activated cell sorting (FACS). ..

    FACS:

    Article Title: Therapy-induced senescence in normal tissue promotes breast cancer metastasis
    Article Snippet: Cyclophosphamide monohydrate was purchased from Sigma-Aldrich (C0768). .. As indicated, tumour cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (Genescript, Sall-IRES-Luc2-XbalXhol_PGK-H2BmCherry); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry+/RFP+ cells were selected by fluorescence-activated cell sorting (FACS). ..

    Article Title: Therapy-induced normal tissue damage promotes breast cancer metastasis
    Article Snippet: .. As indicated, tumor cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2) a firefly luciferase gene, with an mCherry encoding gene (cells denoted -mChLuc2) (PGK-H2BmCherry-IRIS-Luc2; Genscript); or (3) an RFP encoding gene (cells denoted -RFP) (lentiviral vector pDEST/pHIV-H2BmRFP-rfa_verB, a gift from Matthew Smalley, University of Cardiff). mCherry + /RFP + cells were selected by fluorescence-activated cell sorting (FACS). ..



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